Title:
Paper Chromatography Lab
Paper Chromatography Lab
Statement of the problem:
What is the mobile phase on a paper chromatography investigation?
What is the stationary phase in a paper chromatography investigation?
What is meant by the term retention time?
Paper chromatography is an important separation technique that depends on difference in both absorption and solubility.
Hypothesis:
Our hypothesis was H2O. It would be best because it has polarity and water is good.
Materials:
The materials that we used included: 9 strips of chromatography paper, H2O, CH3OH, C3H7OH, C6H14, 24 well plate,water-soluble overhead pens(black, red, green,brown,purple,yellow,blue).
Safety:
We used goggles and aprons. We stayed out of the fumes, and the fume hood. We put the chemicals under the fume hood. We used all of the safety precautions on this lab, and followed the directions.
Procedure:
First, we got on our safety equipment, then we got all of our materials together. Next, we filled up the 24 well plate with H2O, CH3OH, C3H7OH, and C6H14. Then we marked our paper strips with black marker. After that, we put the dots on the piece if paper and put it in the liquids and watched it rise. Our group let the paper sit in the liquids for approximately 30 minutes. We used the different colors to see which one of them would rise the most. After we wrote down all of our data, and finished the lab, we cleaned up and dumped the stuff out and finished the lab procedure.
Results:
During our lab, we figured out that H2O would rise the most with the black. We tried to use the other colors to compare them to the black. The liquid that rose the least was C6H14 because, the polarity is low.
Conclusion:
In conclusion, we found out that H2O was most polar, and rose up the paper quicker than the other solutions.
What is the mobile phase on a paper chromatography investigation?
What is the stationary phase in a paper chromatography investigation?
What is meant by the term retention time?
Paper chromatography is an important separation technique that depends on difference in both absorption and solubility.
Hypothesis:
Our hypothesis was H2O. It would be best because it has polarity and water is good.
Materials:
The materials that we used included: 9 strips of chromatography paper, H2O, CH3OH, C3H7OH, C6H14, 24 well plate,water-soluble overhead pens(black, red, green,brown,purple,yellow,blue).
Safety:
We used goggles and aprons. We stayed out of the fumes, and the fume hood. We put the chemicals under the fume hood. We used all of the safety precautions on this lab, and followed the directions.
Procedure:
First, we got on our safety equipment, then we got all of our materials together. Next, we filled up the 24 well plate with H2O, CH3OH, C3H7OH, and C6H14. Then we marked our paper strips with black marker. After that, we put the dots on the piece if paper and put it in the liquids and watched it rise. Our group let the paper sit in the liquids for approximately 30 minutes. We used the different colors to see which one of them would rise the most. After we wrote down all of our data, and finished the lab, we cleaned up and dumped the stuff out and finished the lab procedure.
Results:
During our lab, we figured out that H2O would rise the most with the black. We tried to use the other colors to compare them to the black. The liquid that rose the least was C6H14 because, the polarity is low.
Conclusion:
In conclusion, we found out that H2O was most polar, and rose up the paper quicker than the other solutions.
Your whole lab is ruined because you dipped your dots causing an unstable mobile phase,you should have got new chromatography strips, this would allow a proper lab to be conducted.
ReplyDeleteBe more specific about your hypothesis. It would be more helpful if you explained your reasons to why water would be most polar.
ReplyDeleteneeds more info
ReplyDeleteNice work with separating information, but what was the main question that you wanted to answer? All and all, nice work.
ReplyDeletewhere is your rubric you were supposed to turn in today??? Thursday
ReplyDeleteVery color full. Good pictures. But change the first paragraph color hard to read
ReplyDeleteNeeds more pictures other then that good job
ReplyDelete